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Fig. 7. Vascular and steroidogenic phenotype observed in the Sf1:Wnt4 transgenic XY gonads. (A) Coelomic blood vessel pattern is affected in Sf1:Wnt4 transgenic testis. Testis from transgenic (a-c) and non-transgenic (NT) (d) 13.5 dpc embryos were stained with an antibody to Pecam. (B) Leydig cell differentiation occurs in Sf1:Wnt4 transgenic testis. Whole-mount in situ hybridisation for Cyp11a1 expression on 13.5 dpc XY gonads from a Sf1:Wnt4 transgenic embryo (left) and a non-transgenic embryo (NT) (right). The transgenic gonads were derived from different integration events (1-4).





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