Fig. 2. skd- or kto-mutant clones distort the DV compartment boundary. (A-H) Third instar wing discs containing skd^T606-mutant clones. Wild-type tissue is labeled with GFP (green; C,D,G,H). ap-lacZ expression, marking the dorsal compartment, is stained with anti-ß-gal (blue; A,D,E,H). Wg protein is stained red (B,D,F,H). Arrows indicate clones that distort the DV boundary, producing a bulge in the Wg stripe and a distortion of the ap expression boundary. (A-D) A clone with dorsal cells in the ventral compartment. (E-H) A clone with ventral cells in the dorsal compartment. (I-J) A kto^T631-clone entirely within the ventral compartment that has distorted the DV boundary (arrow). Wild-type tissue is labeled with GFP (green; J) and stained with anti-ß-gal (red) to show ap-lacZ expression (I,J). (K,L) Second instar wing discs containing skd^T413-mutant clones. Wild-type tissue is labeled with GFP (green; L). Anti-ß-gal (red) staining reflecting caps-lacZ expression (K,L). Arrows indicate dorsal clones that continue to express caps-lacZ. Note that the caps-lacZ transgene is on the same chromosome arm as skd and thus is not present in the wild-type twin spots (asterisks in K and brighter green staining in L).

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