Fig. 7. MAP kinase-Gal4/Vp16 nuclear translocation activity is not upregulated by loss of Egfr, Notch, hedgehog or atonal function. Eye discs are shown; anterior rightwards, dorsal upwards. HS:MG activity detected by the UAS:lacZ reporter in the genetic background of temperature-sensitive mutations for Egfr (A,B), Notch (C) and hh (D) at restrictive temperature, and in ato³ (loss-of-function, antigen positive) clones (I-L). (E-H) In ato³ clones, MAP kinase phosphorylation is lost (dpErk antigen, as previously reported) (Chen and Chien, 1999). Note that reporter gene expression is not de-repressed in any of these genetic conditions.

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