Fig. 4. echinoid and argos act in concert to inhibit Egfr signaling, but through different mechanisms. (A,B) Mutations in argos enhance the echinoid phenotype. (A) Discs homozygous for ed^l(2)k01102 show the multiple R8 phenotype when stained with anti-Atonal antibodies. An example of a pair of R8s is indicated by the arrow. (B) Mutations in argos (argos^W11/argos^W11)further enhance the ed^l(2)k01102 phenotype. (C,D) Same genotypes as in A,B. Enhancement of the multiple R8 phenotype is also apparent in more mature R8 cells as visualized with antibodies against Boss. (E-H) Echinoid-FLAG driven by the actin promoter was overexpressed in clonal patches of tissue using the FLP-out technique (green in F and H; see Materials and Methods). Unlike Argos, overexpression of Echinoid had no effect on the pattern of dpERK phosphorylation (E,F) or Boss expression (G,H).

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