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Fig. 3. Movement of LFY-GFP fusions. Confocal images of GFP fluorescence in
inflorescence apices (A,B,D) and leaf epidermis (C) of 2-week-old
ML1::GLFY (A,C), ML1::GFP:LFY (B) and ML1::LFY:GFP
(D) transgenic plants. GLFY moves several cell layers into the underlying
tissue from the L1 in the apex (A). GFP:LFY shows a similar gradient from the
L1 as GLFY (B). The signal from LFY:GFP, which has the longest moving range,
appears `fuzzy' because of its higher cytoplasmic localization (D). All three
fusions are localized to both the nucleus and cytoplasm in leaf epidermal
cells, and bright spots are sometimes found along the cell wall with GLFY
(shown in C).