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Fig. 5. Restricted lateral protein movement in the Arabidopsis shoot apex.
Confocal images of GFP fluorescence for inflorescence meristems with
surrounding young floral primordia from AG
intron*::NLS:2xGFP (A,D), AG
intron*::2xGFP (B,E) and AG
intron*::GLFY (C,F) plants. The confocal images have been
overlaid with images from the transmitted-light channel for orientation only.
Note that the confocal images are optical sections, which is not true for the
transmitted-light images. The mutated AG sequences in the reporters
activate expression in the shoot apical meristem and the center of young
flowers. Close-up views of stage 3 flowers reveal discrete lateral boundaries
of the GFP signal (D-F). No lateral movement is obvious in any of the three
cases.