Fig. 3. Genetic and molecular analysis of zag-1. (A) Genetic map of zag-1 IV region. (B) Schematic of cosmid F28F9 and subclones. Arrows depict hypothetical gene transcripts and direction of transcription (www.wormbase.org) (+, zag-1 rescue; -, no rescue). Restriction site abbreviations are K (KpnI), P (PstI) and S (SalI). (C) C. elegans and C. briggsae zag-1 genomic structures derived from cDNA and genomic sequences. C. briggsae zag-1 structure is hypothetical and based on a comparison with C. elegans sequences. Black boxes indicate exons, white boxes depict 3' UTR and vertical marks represent CACCT(G) motifs. VISTA server was used for some genomic sequence alignment (Mayor et al., 2000). (D) C. elegans ZAG-1 protein structure. Black boxes depict Zn-finger domains, the dark-gray box represents homeodomain and the light-gray box indicates PLDLT motif.

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