Fig. 4. (A) Alignment of predicted amino acid sequences of ZAG-1 proteins in C. elegans (C. e.) and C. briggsae (C. b.). C. elegans sequence is based on full-length zag-1 cDNA (GenBank Accession Number AY224511). C. briggsae sequence is derived from the gene structure in Fig. 3C. Hyphens indicate gaps inserted to maintain alignment and periods represent identical residues in C. briggsae sequence. The homeodomain and five Zn-finger domains are boxed in gray and black, respectively. PLDLT sequence required for association with CtBP is underlined. Inverted carets mark the Q and W residues altered in zd85 and zd86 and arrows mark the relative positions of conserved splice sites. (B) Alignment of Zn-finger domain sequences from C. elegans ZAG-1, Drosophila ZFH-1, mouse EF1 and mouse SIP1. Modified, consensus C2H2-type Zn-finger sequence is shown on top (, aliphatic amino acid). (C) Alignment of homeodomain sequences from C. elegans ZAG-1, ZC123.3 (ZFH-2-like protein), MEC-3 and LIM-4.

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