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Fig. 6. zag-1 expression pattern. DIC (A) and fluorescence (B) image of zag-1::ZAG-1::GFP embryo with GFP expression in nerve ring nuclei (int., intestinal autofluorescence). (C) zag-1::gfp(zdIs21) (2.9 kb upstream fragment) adult hermaphrodite with expression in subset of nerve ring neurons, no expression in ventral nerve cord neurons. (D) Tail region of zag-1::gfp(zdIs21); zag-1(-918A,-895T)::rfp(zdIs41) adult. zag-1 promoter (4.0 kb) containing mutated CACCT sites directed RFP expression in more neurons than the wild-type promoter (GFP). Both promoters directed expression in anal depressor and intestinal muscles. (E) zag-1(zd86) zag-1::gfp(zdIs21). zag-1 mutations induced expression in ventral cord motoneurons. (F) zag-1(-918A,-895T)::rfp(zdIs41). zag-1 promoter with mutated CACCT sites directed RFP expression in ventral cord motoneurons. Anterior is leftwards (C), anterior is rightwards (D-F), dorsal is towards the top.





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