Fig. 2. Early expression of vhnf1 in the posterior hindbrain. (A) Co-localization of hoxb1b (orange) and vhnf1 (purple). At 100% epiboly, the anterior boundary of vhnf1 expression is posterior to that of hoxb1b. (B) Co-localization of fgf8 (orange) and vhnf1 (purple). At 100% epiboly, fgf8 expression is apparent in the presumptive anterior hindbrain (arrowhead) and in the germ ring (arrow), and there are cells between the posterior boundary of fgf8 and the anterior boundary of vhnf1 that express neither gene. (C) Co-localization of fgf8 (purple) and hoxb1b (orange). At 100% epiboly, fgf8 and hoxb1b domains of expression are adjacent. (D) Co-localization of vhnf1 (orange) and krox20 (purple). At the one-somite stage, vhnf1 expression overlaps the posterior half of r5 krox20. (E,F) Co-localization of vhnf1 (purple) and hoxb1a (orange). At tailbud stage (E), the anterior boundary of hoxb1a expression lies anterior to that of vhnf1 but hoxb1a expression overlaps that of vhnf1 significantly throughout the posterior region. By the two-somite stage (F), hoxb1a expression has resolved to a single stripe and there is no overlap with vhnf1. (A-F) All embryos are dorsal view, anterior to the top. (G) Summary of expression data, anterior to the left.

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