Fig. 7. In situ hybridizations depicting the pattern of FZP expression (arrowheads) during the development of wild-type (A-G) and fzp-2 (H,I) spikelets. (A) Wild-type SAM at the spikelet differentiation stage. FZP RNA (arrowheads) is detected in spikelet meristems before and during the formation of rudimentary glume (RG) primordia. (B) Early stage of spikelet meristem (SM) differentiation. FZP is expressed just above the position where the RG primordium emerges. (C) SM at early inner RG differentiation stage. While the expression of FZP decreases at the outer RG axil, it increases on the iRG side. (D) SM at early palea differentiation stage. FZP expression at the inner RG axil persists weakly until the initiation of the palea primordium. Note that FZP is not expressed at the position of the lemma and palea primordia initiation or in their axils. (Inset) Higher magnification of the region expressing FZP. (E-G) FZP expression extends to a half-ring domain. (E) Lateral view. FZP expression is interrupted around the circumference of the SM. RG primordia (arrows) emerge right under the domain of FZP expression. (F,G) Frontal view of successive transverse sections. FZP is expressed at the sides but not at the center of the SM. (H) Late fzp-2 SAM. FZP is expressed at the tip of secondary branches. (I) Higher magnification of the tip of a secondary branch. FZP is expressed at the axil of a differentiating RG primordium. (J) Schematic representation of the spatial and temporal expression of FZP during the differentiation of a spikelet. org, outer rudimentary glume; irg, inner rudimentary glume; oeg, outer empty glume; ieg, inner empty glume. Scale bars: 100 µm.

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