Fig. 8. Integrins regulate Sog protein distribution on the dorsal wing surface. The distribution of Sog and integrin proteins was examined in wings containing integrin^- clones. In all panels, integrin staining is green and Sog staining is red. mys^- clones were generated to analyze the distribution of Sog protein in the absence of ß-integrin (A-F). Clones generated on the dorsal surface induce a patchy pattern of Sog distribution (A-C). In addition (C), adjacent to a dorsal mys^- clone Sog protein does not enter the provein area (double arrow), while on the opposite side of the same vein, Sog enters the vein competent domain (arrow). Integrin staining defines the limit of the intervein territory (B,C). A ventral mys^- clone does not alter the distribution Sog (DF), while inside a small dorsal and ventral clone, Sog staining is reduced and patchy in appearance. (G-L) mew^- clones result in similar effects on Sog protein distribution. (G-I) A dorsal mew^- clone running between L4 and L5 veins induces patchy Sog distribution. The arrow in I indicates that Sog enters the provein area on the opposite side of the vein. A high magnification confocal optical section localized at the basolateral region of the dorsal wing epithelium reveals that Sog (J,L) and PS1 (K,L) co-localize at the intervein area where the integrin is expressed, while a Sog is distributed in a patchy fashion at the same cell level inside a mew^- clone.

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