Fig. 2. Gradual loss of cardiogenic activity of GATA4. Cardiac tissue can be induced after gastrulation, and even during tailbud stages. (A) GataA4-GR was activated by the addition of dexamethasone (dex) at stage 9, 12, 13.5 or 20. (B) Sox17 is efficiently induced when Gata4 is activated at stage 9, but is induced poorly when it is activated at stage 13. Nkx2.5 and Nkx2.3 are expressed in uninjected animal caps (see also Fig. 5). (C) LFABP is only induced if Gata4-GR is activated at stage 9, but not at stage 13. Two different experiments are shown, and an additional two yielded the same result. Analysis was performed at stage 37/8 (A,C) and stage 18 (B), respectively.

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