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Fig. 6. Centrosome duplication is not affected in fue mutants. Centrosomes at the eight-cell stage during interphase, visualised with an antibody directed against {gamma}-tubulin (green) and nuclei labelled with DAPI (blue) from (A) a wild-type embryo, (B) fue embryo, (C) an embryo treated with the DNA replication inhibitor aphidicolin. (D-F) Enlargements of single centrosome pairs of A-C, respectively. Centrosomes are present and are able to duplicate in all fue cells as well as the (anucleate) cells of aphidicolin-treated embryos, demonstrating that the centrosomes can duplicate independently from nuclei. Scale bars: 20 µm.





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