Fig. 3. blr mutant alleles. (A) Diagram of the BLR gene is with locations of Ds insertions in blr-1 and blr-2, and the T-DNA insertion in blr-3 indicated. (B) RT-PCR amplification using gene-specific primers and hybridization of products with gene-specific probes. BLR transcripts are detected in wild type but not in the three mutants (top panel). RBC transcripts were amplified as a control (bottom panel). (C) Northern hybridization detects low levels of BLR transcript in wild-type seedling and leaf tissue and much higher levels in the inflorescence and flowers. No wild-type transcript is detected in the blr-1 mutant. Lower panel shows an ethidium bromide-stained gel reflecting relative amounts of RNA.

Return to article