Fig. 6. Smo transgenes act cell autonomously to affect Hh responses. UasSmo^high (A), UasSSF (B) and UasSmoC (C) were clonally expressed using the FLP-out Gal4 system and marked by UasGFP (green in all panels). Ptc protein (red in A and B) is normally expressed at high levels just anterior to the compartment border (arrow indicates the border). Overexpression of Smo (green in A') induced high levels of Ptc protein, both near the compartment border and near the anterior edge of the disc. Levels of Ptc protein (red) were reduced when cells along the compartment border expressed SSF (green in B', outlined in white in B). While the levels of Ptc in wild type cells are somewhat variable on a cell-by-cell basis, Ptc levels were distinctly reduced in cells expressing SSF, even in the small clones at the bottom of B, demonstrating a cell-autonomous response to SSF. Ci155 (red in C) accumulated in clones away from the compartment border that expressed SmoC (asterisk in C), as well as in its normal domain paralleling the compartment border.

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