Fig. 1. Loss of PAR-1 causes partially penetrant defects in follicle cell polarity. In all the figures, the follicular epithelium is shown with its apical side (which faces the oocyte) towards the top of the picture and its basal side towards the bottom. (A) Stage 6 egg chamber containing a par-1 mutant clone induced early in oogenesis, marked by the loss of nuclear GFP (in this and all subsequent figures of clones, GFP is shown in the first column, and is shown in green in the merged images in the third column). Mutant cells lose their epithelial organisation, and fail to localise DaPKC apically (centre panel: red in merged image). (B) A stage 10a egg chamber containing a smaller clone induced later in oogenesis, showing normal epithelial organisation and DaPKC localisation. Note that the nuclei are no longer in a consistent position in mutant cells. (C) Stage 9 egg chamber containing three mutant cells stained for the apical marker Neurotactin (Nrt). Most mutant cells in small clones show a wild-type apical localisation of Nrt (top right mutant cell), but some cells show reduced localisation (middle) or no localisation at all (bottom left). (D) Stage 9 egg chamber containing a small mutant clone stained for Notch, which localises apically as in wild type, even when the mutant cells form a double layered epithelium.

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