Fig. 3. MT density is increased in all par-1 mutant clones. (A) A stage 10 egg chamber containing two small par-1 mutant clones, fixed under optimised conditions for preserving MTs (8% PFA) and stained for -Tubulin. The mutant cells contain more MTs than the adjacent wild-type cells. (B) Expression of a GFP-PAR-1 fusion protein rescues this phenotype. The mutant cells that express the transgene can be identified by the presence of lateral GFP-PAR-1 signal and by the loss of nuclear GFP, and show a MT network that is similar to that in the neighbouring wild-type cells. (C) Under standard fixation conditions (4% PFA), par-1 clones appear to lack MTs, suggesting that MTs are less stable than in wild type. (D) Quantification of the intensity of the GFP and the -Tubulin staining in wild-type and mutant follicle cells. Fluorescent signal was measured along the broken white line using the Laser Pix4 software (BioRad). The par-1 mutant cells, which are marked by the decrease in the GFP fluorescence (green line), show twice as much microtubule staining (red line) as the wild-type cells. GFP, green; -Tubulin, red.

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