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Fig. 6. Time-lapse video microscopy of living MG neurons migrating on the midgut. Examples show DiO-labeled MG neurons from embryos staged between 64% and 68% E. Time intervals between the images are 20 minutes. On average, MG neurons migrated 12 µm/hour under normal conditions. To examine the effects of inhibiting sGC on individual neurons, the preparation was incubated in culture medium containing 200 µM ODQ (right). Under these conditions, there was no misrouting of the neurons and they completely stopped migrating. Scale bars: 10 µm.





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