Fig. 3. scd1 mutants display a dwarfed phenotype. (A) scd1-1, scd1-2 and scd1-3 mutants grown on MS-Suc under continuous light for 8 days are smaller, have shorter roots, and have darker green leaves than those of wild type. (B) Mutant shoots are smaller and darker green than wild type. (C) Comparison of wild type, scd1-1 and scd1-2 root cell size. Cell length was plotted as a function of the distance from the root tip. Each point represents an average of 20-50 cell measurements of a mixture of epidermal and cortical cells. (DH) Root hair development in wild type and scd1 mutant seedlings grown vertically on the surface of MS-Suc medium. (D) Stereomicroscope image of surface views of a wild type (WT) and a scd1-2 root. (E-H) Phase contrast images of (E-G) scd1/2 and (H) wild-type roots. scd1 mutants usually form small blebs (D,E,F) at the basal end of trichoblasts, which do not extend. (E) Enlargement of boxed area in D. Arrows in G indicate branched root hairs. Scale bars: 1 cm (A); 0.25 cm (B); 100 µm (D-H).

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