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Fig. 6. RT-PCR analysis of SCD1 expression in wild-type and mutant tissue. RT-PCR analysis of SCD1 mRNA expression in total RNA isolated from wild-type roots, rosette leaves, cauline leaves, immature flower buds, open flowers, scd1-1 leaf tissue, and actively dividing suspension-cultured cells (T87). A reaction (-) containing no DNA template was included as a negative control. RT-PCR amplified ubiquitin was used as a loading control. std: 1.0 and 0.5 kb markers.





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