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Fig. 6. xTsgW67G overexpression prevents dorsalization by LiCl. (A) Untreated stage 30 embryo. (B) Radially dorsalized embryo obtained after LiCl treatment at the 32-cell stage (DAI=9.25, n=16). (C) Embryo microinjected into the marginal zone of each blastomere at the four cell stage with 500 pg xTsgW67G mRNA (DAI=1.1, n=11) or (D) microinjected with a total of 8 ng anti-Chordin morpholino oligos at the two-cell stage (DAI=6.4, n=36) prior to LiCl treatment at 32-cell stage. Note that xTsgW67G causes complete ventralization, which cannot be reversed by LiCl treatment.





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