Fig. 1. The lotus-leaf structure of as1-101 and as2-101 mutants in the Ler genetic background. (A) Ler; (B) as1-101 and (C) as2-101 seedlings. Note that first pairs of rosette leaves in the as1 and as2 mutants often show the lotus-leaf structure (arrows). c, cotyledon. (D-L) Each panel shows one of the first pairs of rosette leaves, all of similar ages, and photos were taken from an adaxial view. (D) A Ler rosette leaf with an asymmetric petiole in the adaxial-abaxial axis. (E,F) as2-101 rosette leaves showing petioles with radially symmetric portions that vary in length (arrowheads). Note that the petiole portions below arrowheads are radially symmetric. (G-I) as2-101 rosette leaves with a varying degree of severity of the lotus-leaf structure. (J) A needle-like structure of an as2-101 seedling. (K) An as2-1 rosette leaf with the radially symmetric portion in the petiole (arrowhead). (L) An as1-1 petiole. Scale bars:1 mm (A-C); 0.2 mm (D-L).

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