Fig. 5. Numb/Notch regulation of NB7-3 development. All panels with the exception of F show segments of the ventral cord immunostained for Ddc (red) and corazonin (green). (A) The wild-type larval pattern shows that corazonin is not expressed in segments A7 and A8. (B) numb¹ mutant embryos show the absence of EW2 and EW3. (C) The UAS-Notch: eg-gal4 larval pattern shows the absence of EW1, EW2 and EW3. (D-F) spdo^G104 mutant embryos show ectopic Ddc-expressing cells, D is a thoracic hemisegment and E shows abdominal segments. (F) A spdo^G104 mutant embryo immunostained for Ddc (red), Zfh-2 (green) and Hb (blue), shows two Ddc cells immunoreactive for Hb and three Ddc cells immunoreactive for Zfh-2. (G) A spdo^G104 heterozygous embryo shows a pattern that is wild type but expression of markers in EW2 and EW3 is incomplete at this stage. (H) numb¹/numb¹:spdo^G104/+ embryos show rescue of the numb¹ mutant phenotype. Arrows indicate hemisegments that have two dc-expressing cells, arrowheads indicate hemisegments with no Ddc-expressing cells (indicative of the numb phenotype) and asterisks mark the midline dopamine cells. E and L indicate whether the image is from a stage 17 embryo or third instar larva, respectively. M indicates the midline. Scale bar: in B, 15 µm for B,G,H; in C, 15 µm for A,C; in D, 10 µm for D; in F is 3 µm for F; in E, 10 µm for E.

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