Fig. 7. Notch-mediated apoptosis in the NB7-3 lineage. (A-D) Abdominal segments of the ventral cord showing immunoreactivity of Ddc (red) and corazonin (green). E and L indicate whether the image is from a stage 17 embryo or a larva, respectively. M indicates the midline. (A) A eg-gal4/UAS-p35 third instar larval nerve cord shows a wild-type pattern of cells plus ectopic corazonin-containing cells marked with arrowheads. (B) A numb¹/numb¹:eg-gal4/UAS-p35 embryos show evidence that p35 can rescue the numb¹ mutant phenotype. Arrows indicate hemisegments that have two Ddc-expressing cells, and the arrowheads marks a hemisegment with ectopic corazonin-containing cells. A8 shows two Ddc-expressing cells. (C) A UAS-Notch^ACT/+:eggal4/UAS-p35 third instar larval nerve cord has a pattern identical to wild type. (D) A en-gal4/+:UAS-p35/+ third instar larval CNS shows two abdominal hemisegments with triplets of Ddc-expressing cells. (E-E') An entire spdo/+ first instar larval CNS showing overproliferation of Ddc expressing cells. E' is a DIC image of the same CNS as in E, showing the top surface and a protrusion that matches the largest area of cell proliferation in E. Scale bars: in C, 15 µm for A-C; in D, 5 µm for D; in E', 30 µm for E,E'.

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