Fig. 4. Expression of dve during normal wing development. Wing imaginal discs were stained for Wg (red) and Dve (green). Anterior is to the left; ventral to the bottom. (A-C) A wing imaginal disc of the late second larval instar stage. Wg is expressed in a ventral area of the disc (B). At this time, Dve is not expressed (A,C). (D-F) A wing imaginal disc of the early third larval instar stage. Wg expression (E) resolves into a ring-like domain that is bisected by a stripe of Wg expression along the future DV compartment boundary (arrow). At this time, expression of Dve is initiated (D) and is observed in the region that is framed by the ring-like domain of Wg expression (F). (G-I) A wing imaginal disc of the late third larval instar stage. Wg is expressed in two ring-like domains and along the DV boundary (arrow and arrowhead in H). During this phase, Dve continues to be expressed in the area framed by the inner ring-like domain of wg expression (G,I). Note that Dve expression is suppressed at the DV boundary, with exception of a short stretch at the anterior side (arrowhead in G). Dve expression is further lowered in the region of the primordia of wing veins 3 and 4 (arrows in G). (J) No specific staining is observed in dve^P1738-mutant wing imaginal discs of the third larval instar stage following anti-Dve antibody staining. This suggests that in dve^P1738 is a null allele of the locus for wing development. (K) Expression of ß-Gal in the disc shown in J.

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