DEV00630 Movies

Files in this Data Supplement:

• Movie 1 - spn-E prevents premature cytoplasmic streaming. Time-lapse imaging of autofluorescent particles in spn-E^hlsD157/TM3 (WT; movie 1), spn-E^2A9–14/spn-E^hlsD157 (spn-E^2A9–14; movie 2), spn-E^4E2–14/spn-E^hlsD157 (spn-E^4E2–14; movie 3) and spn-E^8D4–11/spn-E^hlsD157 (spn-E^8D4–11; movie 4). Whereas wild-type oocytes show disorganised cytoplasmic movements, 40-60% of spn-E oocytes display a fast circular movement. Each movie represents 15 minutes of filming, lasts 66 seconds (speeding factor: 13.6) and is looped three times.

• Movie 2 - yps and orb act antagonistically to prevent premature cytoplasmic streaming. Time-lapse imaging of autofluorescent particles in yps^JM2 orb^F303/TM3 (WT; movie 1), orb^mel/yps^JM2 orb^F303 (orb; movie 2) and yps^JM2 orb^mel/yps^JM2 orb^F303 (orb yps; movies 3 and 4). Whereas wild-type oocytes show disorganised cytoplasmic movements, orb mutant oocytes display a fast circular movement. Two different oocytes are shown for orb yps double mutants, to indicate that yps suppresses the orb phenotype in half of the oocytes.