Fig. 1. Localisation of GFP-Staufen. (A-C) Expression of GFP-Staufen (green) in fixed egg chambers counterstained with rhodamine-phalloidin, which labels F-actin (red). (A) Stage 6 egg chamber, showing the accumulation of GFP-Staufen in the oocyte. (B) Stage 9 egg chamber, showing the localisation of GFP-Staufen to the posterior of the oocyte. (C) Stage 10b egg chamber, in which GFP-Staufen localises both to the anterior and the posterior pole of the oocyte. (D-F) Localisation of GFP-Staufen in stage 9 egg chambers. (D) Wild type. (E) btz². (F) grk^2B6/grk^2E12. (G,H) Localisation of GFP-Staufen in stage 11 egg chambers. (G) Wild type. (H) exu^VL/exu^QR. Note that the posterior localisation of GFP-Staufen is unaffected. Anterior is to the left, posterior to the right in this and all subsequent Figures.

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