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Fig. 4. Classes of mutants affecting the localisation of bicoid mRNA. GFP-Staufen (green) and rhodamine-phalloidin staining (red) are shown in the left panels (A,C,E). bicoid in situ hybridisations are shown on the right (B,D,F). (A,B) Wild-type egg chambers. (C,D) 7D8-15 egg chambers, an example of the class of mutants in which bicoid mRNA fails to concentrate along the anterior cortex in early stages of oogenesis. (E,F) larsen2B6-3, a member of the second class of bicoid-specific mutants, in which bicoid mRNA localisation in early stages is indistinguishable from wild type, but GFP-Staufen fails to concentrate at the anterior cortex of stage 12 oocytes.





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