Fig. 6. Other phenotypes of mutants found in the screen. (A) sorcière^4E5-2. In this mutant, the bulk of GFP-Staufen (green; top inset) is blocked in the nurse cells and co-localises with the ring canals stained with rhodamine-phalloidin (red; bottom inset). GFP-Staufen also fails to accumulate to the anterior of the oocyte in later stages. (B) trou^9E4-15 shows a defect of adhesion between the posterior follicle cells and the oocyte. This mutant is also defective in bicoid and oskar mRNA localisation. (C) In sedov^9E9-3, a member of the class of mutants in which oskar mRNA is diffusely localised in the posterior part of the oocyte, the organisation of the follicle cells is aberrant. This phenotype may reflect a premature migration of `centripetal' follicle cells between the oocyte and the nurse cells. Alternatively, it may indicate an overproliferation of follicle cells. A similar phenotype was observed in wellman and vagabond mutants. (D) nain^4A3-6 is a member of the class of mutants in which the oocyte fails to acquire wild-type size. The rare oocyte escapers that grow fail to localise GFP-Staufen in a wild-type manner. (E) 4B7-11, one of the two mutants in which the egg chamber contains more than 16 germ cells.

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