Fig. 3. Dll3 and Mesp2 are required for normal expression of each other. In the wild-type embryo at 9.5 dpc, expression of Dll3 is finally localized to the rostral half of each somite (A). The Dll3 stripe (arrowhead in A) is missing in the Dll3^pu/pu embryo (B). The level of Mesp2 expression is significantly decreased in the Dll3^pu/pu embryo (C,D). In the Mesp2-null embryo, a weak diffuse Dll3 expression is expanded rostrally (E,F). (G-J) Expansion of Dll3 expression in the Mesp2-null embryo does not require Dll3. At 11.5 dpc, in the Dll3^pu/pu embryo, the Dll3 stripe is missing and the expression is not expanded rostrally (G,H). Expansion of Dll3 expression in the Mesp2-null embryo is not largely affected by the loss of Dll3 (I,J). (K,L) Dll3 is required for localization of Mesp2 expression into the rostral half of somites. In the wild type, ß-gal activity for Mesp2-lacZ is localized in the rostral half of somites (K). A randomized salt-and-pepper pattern is observed in the Dll3^pu/pu embryo (L).

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