Fig. 4. Effects of morpholino injections on the pattern of genes expressed in the PSM. (A-G) The wild-type pattern, (H-N) embryos injected with her1^mo and (O-U) embryos injected with her7^mo. Two different morpholinos were used in each case (see Materials and Methods), which caused identical effects, respectively. The embryos are between the 8- and 12-somite stage. The columns represent the different probes that were used for in situ hybridisation. her1_ex refers to a cDNA (exon) probe, her1_in to the intron probe. deltaC and deltaD are abbreviated. Two columns are shown for the her1_in and her7 probes, to show different stages of the dynamic expression. Note that the her1_in probe yields a much weaker signal and requires about 15-fold longer staining times than the exon probe (1 hour compared to approximately 15 hours). Moreover, it leads often to additional apparently random punctate expression in the PSM, which is of unknown significance. The embryos that are selected here have relatively few such additional punctate signals - some of the other embryos in the same preparations did show a higher number of these irregular signals. In total 185, 190, 88 and 84 her1^mo-injected embryos from independent experiments were hybridised with antisense riboprobes of her1, her7, deltaC and deltaD, respectively. The penetrance of the observed effects was 92%, 91%, 94% and 90%, respectively. In total 183, 163, 89 and 82 her7^mo-injected embryos from independent experiments were hybridised with antisense riboprobes of her1, her7, deltaC and deltaD, respectively. The penetrance of the observed effects was 96%, 92%, 91% and 91%, respectively. In total 230 her1^mo-injected embryos were hybridised with her1 intron probe. The observed penetrance in 3 independent experiments was 92%, 96% and 93%, respectively. All in dorsal view, anterior to the top, flat-mounted embryos.

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