Fig. 6. Soluble SDF1 enhances PGC survival in E10.5 slices. (A) A control slice before culture. This slice was optically sectioned and the picture shown is a projection (z=156.4 µm). Red marks indicate counted cells. (B) The slice shown in A after 20 hours in culture. The picture shown is a projection (z=83.7 µm). Slices compress while in culture. (C) An SDF1-treated slice prior to culture (z=150.0 µm). (D) The slice shown in C after 20 hours in the presence of 500 ng/ml SDF1 (z=76.8 µm). (E) Summary of survival data from two culture experiments. The slice shown in A is Control 3 in the graph, and the slice shown in C is SDF1 5. Most control slices have poor survival, whereas SDF1 treatment enhances survival and/or proliferation. Scale bar: 55 µm (A-D).

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