Fig. 2. GP130 is expressed in the adult ovary and testis. (A) Control. (B) Primordial follicle stained with gGP130. Staining is weak and diffuse in the oocyte. (C) Growing follicle stained with gGP130. Cell surface staining is evident at the surface of the oocyte, and in the thecal and granulosa cells of the follicle. (D) Seminiferous tubule stained with naïve goat IgG. Staining at the periphery of the tubule and in Leydig cells is nonspecific. (E) Seminiferous tubule stained with gGP130. This tubule contains elongating spermatids but no mature sperm. Cell surface staining is most prominent in the primary spermatocytes, which also exhibit a strong perinuclear spot of GP130 staining. (F) Seminiferous tubule stained with gGP130. This tubule contains mature sperm. GP130 is strongly expressed on the surface of all spermatocytes and spermatids. (G,H) The M20 antibody also stains growing oocytes (G) and primary spermatocytes (H); however, staining is more diffuse. Scale bars: 20 µm for A,D,E,G,H; 10 µm for B,C,F.

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