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Fig. 5. Ovaries from GCKO females exhibit no gross morphological defects. (A,B) Hematoxylin and Eosin stained sections from a GCKO ovary (A) and a GP130Flox/GP130+ Cre+ sibling (B). Follicles at various stages are evident. Primordial (p), primary (pr), growing (g), atretic (a) and antral (an) stage follicles are indicated. (C) GCKO females (n=6) have normal numbers of total follicles relative to their siblings (n=5). Follicles were counted in every fifth section. (D) Counted follicles were staged based on morphology. GCKO females have a slight, but statistically significant reduction in the percentage of follicles in the primary stage (Student's t-test, P<0.03). Error bars show standard deviations. Scale bars: 30 µm.





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