Fig. 1. Tissue-specific expression of a GFPKN1 fusion produces transgenic plants carrying distinct phenotypes. (A) Plants expressing mGFP5-ER in the mesophyll had a normal phenotype. (B,C) Epidermis-specific expression of GFPKN1 using pLTP1 (B) or pAtML1 (C) resulted a mildly rumpled leaf phenotype. (E) Mesophyll-specific GFPKN1 expression resulted in lobed leaf phenotypes (D) that were reminiscent of plants overexpressing KNOX genes using the 35S promoter. (E) Representative leaves of plants shown in A-D are displayed in order. (F,G) Severe pRbcS2b-GFPKN1 seedlings: (F) SEM and (G) a confocal image showing an ectopic shoot on the adaxial side of the leaf (G). Scale bars: 1 cm (A-E), 1 mm (F), 50 µm (G).

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