Fig. 4. GFP fusions to KNAT1, STM and KN1 are able to traffic in the inflorescence SAM. All reporter constructs were expressed under the control of pAtML1. (A,B) Cell-autonomous mGFP5-ER (A) and GUSGFP (B) reporter expression demonstrates the L1 specificity of pAtML1. The high magnification views (A-B, insets) show predominant perinuclear or cytoplasmic green fluorescence of mGFP5-ER or GUSGFP. (C-E) The GFP fusions to KN1 (C), STM (D) and KNAT1 (E) showed strong L1 and weaker L2 green fluorescence suggesting short-range movement. (F,G) GFPKNAT1 is detected in L3 as well as L1 and L2 in the two-photon microscope image (F), and is quantified (G) in the region corresponding to the red-box in F. Nuclear localization of GFP fusions to KNOX proteins is also evident in the cross section images in L1 and L2 cells (C-F, arrowheads). L1 expression of GFPTVCV MP (H) or GFP (I) resulted in more extensive movement in the SAM than the GFPKNOX fusions. Scale bars: 25 µm (A), 50 µm (B-I), 10 µm (insets).

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