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Fig. 5. L1-specific expression of KN1, STM or KNAT1 is sufficient to partially complement stm-11. (A) GUS expression from the STM promoter (pSTM) was detected mainly in the peripheral region of the SAM. (B) pSTM driven GFP~KN1 expression shows a similar pattern to that of GUS, but with GFP fluorescence also in the central region (arrowhead). (C) The pSTM-GFP~KN1 transgene complemented stm-11, producing stm homozygous plants with shoots showing relatively normal phyllotaxy and flower morphology (inset) (an stm-11 seedling with fused cotyledons and no true leaves is shown in M, upper left). (D) L1-specific expression of KN1 (left) and GFP~KN1 (right) under the control of pAtML1 partially rescued stm-11. (E) pAtML1-GFP~STM (or STM) expression resulted in a severe phenotype in most plants, although some of the partially rescued stm-11 homozygous plants had a bushy phenotype (F). (G) L1-specific GFP~KNAT1 expression could also partially rescue stm-11. (H-K) The GUS~KN1 fusion protein was cell-autonomous in the leaf (H-J) and in the SAM (K). Perivascular-specific GUS staining of the leaf of a J2111/UAS-GUS~KN1 plant seen from the top down (H) and in cross section view (I). (J,K) In pAtML1-GUS-KN1 plants, GUS activity was epidermis specific in the leaf (J) and in the shoot apex (K). The inset in K shows epidermal GUS~KN1 expression in a whole-mount stm-11 seedling. (L,M) In 35S-GUS~KN1 plants, GUS activity was detected in all SAM layers (L), and 35S-GUS~KN1 could partially rescue stm-11; two rescued stm-11 seedlings (right), stm-11 seedling (top left) and wild-type seedling (bottom left) are shown in M. However, over-expression of GUS~KN1 in the leaf does not lead to over-expression phenotypes. (N,O) A J2111/UAS-GFP~KN1 plant shows KN1 over-expression phenotypes (N), while J2111/UAS-GUS~KN1 plants had wild-type morphology (O). (P) Similarly, 35S-GUS~KN1 overexpressing plants (stm-11/STM genotype) did not show any KN1 over-expression phenotypes, despite showing high GUS activity (inset). Scale bars: 50 µm (A,B,H-L), 0.5 cm (F and insets in K,P), 1 cm (E,G,M-P), 3 cm (C,D).





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