Fig. 1. Crb is required for Sdt localization at rhabdomere stalk. (A) A schematic representation of photoreceptor cell in pupal stage. Crb and Dlt colocalize specifically to the rhabdomere stalk between the rhabdomeres emerging from the apical surface and the AJs. Confocal images shown in B-E were sectioned as indicated by the broken line. (B-E) Co-localization of Dlt and Sdt in photoreceptors at 40% pd. Dlt (green) is localized in apical membrane in the most central region of a photoreceptor cluster whereas AJ (red) is immediately basal to the Dlt domain (B). Later, rhabdomeres form apical to the Dlt domain, which will become the rhabdomere stalk between rhabdomere and AJ. Dlt (C, red) and Sdt (D, green) are colocalized in the rhabdomere stalks. Both proteins show extensive colocalization (E). (F-N) Dlt (F,I,L; red) and Sdt (G,J,M; green) are mislocalized in the crb^- photoreceptors cell autonomously. In crb^- mutant cells identified by the absence of GFP staining (H,K,N; blue), Dlt and Sdt are co-mislocalized (H,K,N). Mislocalization of Dlt and Sdt in crb^- mutant cells is relatively mild in the earlier pupal eyes (F-H; 25% pd), but becomes more severe in the later pupal eyes (I-K; 35% pd) and then completely diffused to basolateral membrane (L-N; 50% pd).

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