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Fig. 7. Pph13 is required for the expression of photoreceptor specific genes. (A-C) Western analysis of photoreceptor specific proteins in cellular extracts isolated from cn bw and Pph13hazy adult heads. There is no detection of TRPL, Arr1, TRP{gamma} and eye Gß in Pph13hazy mutant extracts. Both NinaC isoforms (p132 and p174) are shown. (D) Immunofluorescence of photoreceptor specific proteins of 1 µm frozen sections of light-exposed cn bw and Pph13hazy eyes. INAD and TRP localize to the rhabdomeres in both wild-type and mutant cells. TRPL and eye Gß are not detected in Pph13hazy mutants. Arr1 expression is only detected in the inner photoreceptor cells (R7, R8, arrow) and rhodopsin (Rh1) is detected only in the six outer photoreceptor cells (R1-R6).





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