Fig. 8. eye Gß is a transcriptional target of Pph13. (A) Reverse transcriptase reactions coupled to PCR for the detection of photoreceptor specific mRNAs. Transcripts for trpl and eye Gß are not detected in Pph13^hazy mutants. (B) Potential Pph13 binding sites in the eye Gß enhancer and electrophoretic mobility shift assay. Full-length Pph13 binds specifically to the palindromic sites located in the eye Gß enhancer. Arrow indicates shifted complex and asterisks denote the mutated base pairs. W, wild-type enhancer; M, mutated enhancer. (C) Transient transfection assay. The bar graph shows the data from one experiment that is representative of all the transient transfection experiments performed in this study. Each point represents the average of 16 transfected wells (error bar indicates s.d. of each data set). (D) Western analysis of eye Gß-GFP expression in wild-type and mutant Pph13^hazy flies. Protein extracts were isolated from both heads and bodies of wild-type and Pph13^hazy mutant flies that contained two copies of the eye Gß enhancer driving GFP expression. Antibodies against G were used as a loading control for the head extract lanes.

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