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Fig. 2. Colony morphology and analysis of hemoglobin mRNA expression. Photomicrograph of an E8.5 yolk sac primitive erythroid cells (EryP) and a definitive erythroid progenitor cell (BFU-E). Magnification x100. Panels to the right of the photomicrographs depict the results of RT-PCR analysis of plucked erythroid colonies and reveal that EryP contain mRNA for ßH1 (embryonic hemoglobin) and ß globin major (adult hemoglobin) while BFU-E express mRNA for only ß globin major. Negative control samples were tested using no reverse transcriptase.





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