Fig. 8. Abi binds Kette and Wasp. The yeast two hybrid system was used to determine the interaction between Kette and Wasp. The indicated constructs were tested for their ability to induce three distinct selection genes (-Ade, -His and -Gal reporters). The interactions of Kette and Abi and Wasp and Abi reconstitute functional GAL4 proteins, which enable yeast cells (AH109 strain) to grow on plates lacking adenine and histidine (Ade^-, His^-). In addition, these interactions activated -Gal expression as demonstrated by the blue color of the colonies. Yeast cells co-transformed with the Gal4 fusions pGAD-Kette and pGBK-Kette; pGAD-Kette and pGBK-Wasp failed to grow on selection plates.

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