Fig. 3A. MMP9 expression, fibrin accumulation, and vascularization in implantation sites from galardin-treated wild-type and Plg-deficient mice. (A) At 7.5 dpc in situ hybridization for MMP9 mRNA showed expression in the giant trophoblast cells (black arrows). Few giant trophoblasts were observed in the ectoplacental cone in 7.5 dpc implantation sites from galardin-treated Plg-deficient mice (part d) compared with implantation sites from mock- and galardin-treated wild-type mice and from mock-treated Plg-deficient mice (parts a-c). Fibrin(ogen) was detected by immunohistochemical staining using rabbit anti-fibrin(ogen) (parts e-h). No difference in the fibrin(ogen) staining pattern was observed among implantation sites from the four groups of mice. Endothelial cells were detected by in situ hybridization for PECAM mRNA (parts i-l). A decrease in the vascularization was seen in implantation sites from some galardin-treated Plg-deficient mice (part l), when compared with implantation sites from mock- and galardin-treated wild-type mice and from mock-treated Plg-deficient mice (parts i-k) (see Table 2). The areas boxed in parts i and l are shown at higher magnification in parts m and n, respectively. am, antimesometrial; d, decidua; e, embryo; m, mesometrial. Scale bar: 500 µm in parts a-l; 50 µm in parts m,n.

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