Fig. 4. Phenotypic consequences of ectopic eyg and toe expression. The expression of the different Gal4 lines driving UAS-LacZ or UAS-GFP is shown in the insets. (A) Notum of a fly of genotype 248-Gal4 > UAS-eyg. The scutellum is transformed toward scutum as indicated by the change of morphology and the presence of microchaetes in the posterior notum. Note the opposite polarity of the bristles in the transformed and untransformed territories. (B) Transformation observed in the notum of 248-Gal4 > UAS-toe flies, resembling that portrayed in A. (C) Notum of a 638-Gal4 > UAS-toe fly. The ectopic expression of toe is limited to the scutellum region (arrow in inset), which differentiates microchaetes (arrow). (D) Similar transformation found in 455-Gal4 > UAS-eyg. The driver directs expression to the scutellum region (inset). (E) The broader expression (inset) of eyg in the posterior notum region produces more extensive transformation. (F) In ap-Gal4 > UAS-eyg flies the Eyg product is uniformly expressed in all the notum cells. It results in an expansion of the normal anterior-central region of the notum, and the disappearance of the scutellum and the lateral region.

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