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Fig. 3. Structure-function analysis of Kek1. (A) Schematic representation of the different Kek1 constructs: SP, signal peptide; NT, N-terminal region; LRR, Leucine-Rich Repeat domains; Ig, Immunoglobulin-like domain; TM, transmembrane domain. The relative efficiencies of these truncated proteins to inhibit DER signalling after overexpression in the follicle cells and the wing imaginal discs are indicated. (B) In vitro association between Kek1 and DER. Sf9 cells were infected with baculovirus encoding DER and co-infected with nothing (lane 1) or viruses encoding Myc-tagged versions of wild-type Kek1 (lane 4), Kek1{Delta}LRR (lane 2), Kek1{Delta}Ig (lane 3). Lane 5 is a control with Sf9 infected with baculovirus encoding Kek1-Myc alone. Anti-DER and anti-Myc immunoprecipitates, lane 1 and lane 2-5, respectively, were blotted with anti-DER (upper panel) and then reprobed with anti-Myc (lower panel).





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