Fig. 8. Inhibition of mammalian cell growth by Kek1. (A) Inhibition of anchorage-dependent cell growth. The growth rate of cells stably transfected with vector alone (v.o.) or cells transfected with epitope-tagged Kek1 were compared for MDAMB-468 human mammary tumor cells, and NF-639, IJ9921 and AC-816 mouse mammary tumor cells. Growth rates of HEK293 cells treated without and with Kek1 induction by PonA were also compared. Experiments were carried out in triplicate and repeated at least three times. (B) Inhibition of tumorigenic growth properties by Kek1. The growth of cells in soft agar or as tumors in nude mice was compared. Plotted is the percent inhibition by Kek1 transfectants relative to vector alone transfectants. Error bars represent the standard error of the mean of three to six determinations. Experiments were repeated at least three times.

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