Fig. 6. Subcellular localization of activated ERK in mouse embryos. (A,E,I,M) Whole-mount views of dp-ERK stained embryos at 10.5, 8.5, 9.0 and 6.5 dpc, respectively. High magnification confocal and deconvolution sections of these dp-ERK stained embryos reveal cytoplasmic subcellular localization in endogeneous signaling domains such as limb bud mesenchyme (B-D) and forebrain neural ectoderm (F-H), but cytoplasmic and nuclear staining in mitotic cells (N-P) and regions of injury (J-L). (B-D,F-H,J-L) Confocal sections of dp-ERK/YOYO1 stained embryos in the regions indicated in A,E,I, respectively. (N-P) Deconvolution images of a dp-ERK/Hoechst-labeled embryo sectioned transversely at the position indicated in M. Arrowheads indicate mitotic dp-ERK positive cells. Arrows indicate non-mitotic cells that exhibit both cytoplasmic and nuclear dp-ERK staining in the region of torn somites. Scale bars: 700 µm in A; 250 µm in E; 400 µm in I; 75 µm in M.

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