Fig. 6. The human RFX4 locus and its three known transcripts. This figure is a schematic representation of 200 kb of human genomic sequence from NT_009720.8, shown in reverse complement orientation, and of the position within this sequence of the exons that comprise the three indicated RFX4 transcripts. At the top of the figure is shown the transcript corresponding to RFX4_v2 (Accession Number NM_002920). Exon 1 is unique to this transcript (green hatching); exons 2-5 (solid green) are shared with the novel RFX4_v3 transcript described in this paper; exons 6-15A (yellow) are shared with the RFX4_v3 transcript as well as the transcript RFX4_v1; exon 15B (green hatching) is apparently unique to this transcript, and contains a polyadenylation sequence and presumably a polyA tail, as indicated by the wavy line. The location of these exons on the genomic sequence are indicated. Below the genomic sequence is represented the transcript RFX4_v1. It contains a unique exon 1 (red hatching); exons 2-11 (yellow) shared with both RFX4_v2 and RFX4_v3; and exons 12-14 (red) shared only with RFX4_v3. The RFX4_v3 transcript contains a unique exon 1 (purple); exons 2-5 (green) shared only with RFX4_v2; exons 6-15 (yellow) shared with both RFX4_v1 and RFX4_v2; and exons 16-18 (red) shared only with RFX4_v1. The site of transgene insertion is indicated in the genomic clone by the black X in the intron between exons 13 and 14 of RFX4_v1; its position between exons 17 and 18 of RFX4_v3 is also indicated. The regions of the RFX4_v3 transcript coding for the 737 amino acid human RFX4_v3 protein (blue) are indicated, as is the DNA-binding domain (DBD) of the protein. See the text for additional details.

Return to article