Fig. 1. Expression patterns of the regulatory elements contained in the aex-3 and myo-3 transgenic constructs. Left-hand images are Nomarski, and right-hand images are fluorescence. Scale bars: 20 µm. (A,B,G,H) Body-wall muscle. (C,D,I,J) Ventral cord neurons. (E,F,K,L) Vulval precursor cells. (A-F) Neuronal-specific expression of yfp from the aex-3 promoter in the presence of the last intron from egl-30. Animal is an early L3 stage hermaphrodite containing the pha-1(e2123ts) mutation, and rescued by the extrachromosomal array syEx570 [pha-1, aex-3::yfp::egl-30]. (B,F) Background fluorescence is from neuronally expressed YFP in other focal planes. (G-L) Muscle-specific expression of the egl-19 genomic coding region fused to gfp from the myo-3 promoter. Animal is an early L3 stage hermaphrodite containing the egl-19(n582) mutation and the extrachromosomal array syEx476 [myo-3::egl-19::gfp]. (J,L) Background fluorescence is from body-wall muscle-expressing GFP in other focal planes. AC, anchor cell.

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